Vitamin B6

Pyridoxine

Pyridoxal phosphate

Vitamin B₆ is a water-soluble vitamin and is part of the vitamin B complex group. Pyridoxal phosphate (PLP) is the active form and is a cofactor in many reactions of amino acid metabolism, including transamination, deamination, and decarboxylation. PLP also is necessary for the enzymatic reaction governing the release of glucose from glycogen.

History

Vitamin B₆ is a water-soluble compound that was discovered in the 1930s during nutrition studies on rats. In 1934, a Hungarian physician, Paul Gyorgy discovered a substance that was able to cure a skin disease in rats (dermititis acrodynia), this substance he named vitamin B₆. In 1938, Lepkovsky isolated vitamin B₆ from rice bran. Harris and Folkers in 1939 determined the structure of pyridoxine, and, in 1945, Snell was able to show that there are two forms of vitamin B₆, pyridoxal and pyridoxamine. Vitamin B₆ was named pyridoxine to indicate its structural homology to pyridine. All three forms of vitamin B₆ are precursors of an activated compound known as pyridoxal 5'-phosphate (PLP), which plays a vital role as the cofactor of a large number of essential enzymes in the human body.

Enzymes dependent on PLP focus a wide variety of chemical reactions mainly involving amino acids. The reactions carried out by the PLP-dependent enzymes that act on amino acids include transfer of the amino group, decarboxylation, racemization, and beta- or gamma-elimination or replacement. Such versatility arises from the ability of PLP to covalently bind the substrate, and then to act as an electrophilic catalyst, thereby stabilizing different types of carbanionic reaction intermediates.

Overall, the Enzyme Commission has catalogued more than 140 PLP-dependent activities, corresponding to ~4% of all classified activities.

Forms

Seven forms of this vitamin are known:

• pyridoxine (PN). PN is the form that is given as vitamin B₆ supplement.
• pyridoxine 5'-phosphate (PNP).
• pyridoxal (PL).
• pyridoxal 5'-phosphate (PLP). PLP is the metabolically active form.
• pyridoxamine (PM).
• pyridoxamine 5'-phosphate (PMP).
• 4-pyridoxic acid (PA). PA is the catabolite which is excreted in the urine.

All forms except PA can be interconverted.

Functions

Pyridoxal phosphate, the metabolically active form of vitamin B₆, is involved in many aspects of macronutrient metabolism, neurotransmitter synthesis, histamine synthesis, hemoglobin synthesis and function and gene expression. Pyridoxal phosphate generally serves as a coenzyme for many reactions and can help facilitate decarboxylation, transamination, racemization, elimination, replacement and beta-group interconversion reactions. The liver is the site for vitamin B₆ metabolism.

Amino acid metabolism

Pyridoxal phosphate (PLP) is a cofactor in transaminases that can catabolize amino acids. PLP is also an essential component of two enzymes that converts methionine to cysteine via two reactions. Low vitamin B₆ status will result in decreased activity of these enzymes. PLP is also an essential cofactor for enzymes involved in the metabolism of selenomethionine to selenohomocysteine and then from selenohomocysteine to hydrogen selenide. Vitamin B₆ is also required for the conversion of tryptophan to niacin and low vitamin B₆ status will impair this conversion. PLP is also used to create physiologically active amines by decarboxylation of amino acids. Some notable examples of this include: histidine to histamine, tryptophan to serotonin, glutamate to GABA (gamma-aminobutyric acid), and dihydroxyphenylalanine to dopamine.

Gluconeogenesis

Vitamin B₆ also plays a role in gluconeogenesis. Pyridoxal phosphate can catalyze transamination reactions that are essential for the providing amino acids as a substrate for gluconeogenesis. Also, vitamin B₆ is a required coenzyme of glycogen phosphorylase, the enzyme that is necessary for glycogenolysis to occur.

Lipid metabolism

Vitamin B₆ is an essential component of enzymes that facilitate the biosynthesis of sphingolipids. Particularly, the synthesis of ceramide requires PLP. In this reaction serine is decarboxylated and combined with palmitoyl-CoA to form sphinganine which is combined with a fatty acyl CoA to form dihydroceramide. Dihydroceramide is then further desaturated to form ceramide. In addition, the breakdown of sphingolipids is also dependent on vitamin B₆ since S1P lyase, the enzyme responsible for breaking down sphingosine-1-phosphate, is also PLP dependent.

Metabolic functions

The primary role of vitamin B₆ is to act as a coenzyme to many other enzymes in the body that are involved predominantly in metabolism. This role is performed by the active form, pyridoxal phosphate. This active form is converted from the two other natural forms founds in food: pyridoxal, pyridoxine and pyridoxamine.

Vitamin B₆ is involved in the following metabolic processes:

• amino acid, glucose and lipid metabolism
• neurotransmitter synthesis
• histamine synthesis
• hemoglobin synthesis and function
• gene expression

Amino acid metabolism

Pyridoxal phosphate is involved in almost all amino acid metabolism, from synthesis to breakdown.

1. Transamination: transaminase enzymes needed to break down amino acids are dependent on the presence of pyridoxal phosphate. The proper activity of these enzymes are crucial for the process of moving amine groups from one amino acid to another.

2. Transsulfuration: Pyridoxal phosphate is a coenzyme needed for the proper function of the enzymes cystathionine synthase and cystathionase. These enzymes work to transform methionine into cysteine.

3. Selenoamino acid metabolism: Selenomethionine is the primary dietary form of selenium. Pyridoxal phosphate is needed as a cofactor for the enzymes that allow selenium to be used from the dietary form. Pyridoxal phosphate also plays a cofactor role in releasing selenium from selenohomocysteine to produce hydrogen selenide. This hydrogen selenide can then be used to incorporate selenium into selenoproteins.

4. Vitamin B₆ is also required for the conversion of tryptophan to niacin and low vitamin B₆ status will impair this conversion.

Neurotransmitter synthesis

Pyridoxal phosphate-dependent enzymes play a role in the biosynthesis of four important neurotransmitters: serotonin, epinephrine, norepinephrine and gamma-aminobutyric acid. Serine racemase, which synthesizes the neuromodulator D-serine, is also a pyridoxal phosphate-dependent enzyme.

Histamine synthesis

Pyridoxal phosphate is involved in the metabolism of histamine.

Hemoglobin synthesis and function

Pyridoxal phosphate aids in the synthesis of heme and can also bind to two sites on hemoglobin to enhance the oxygen binding of hemoglobin.

Gene expression

It transforms homocysteine into cistation then into cysteine. Pyridoxal phosphate has been implicated in increasing or decreasing the expression of certain genes. Increased intracellular levels of the vitamin will lead to a decrease in the transcription of glucocorticoid hormones. Also, vitamin B₆ deficiency will lead to the increased expression of albumin mRNA. Also, pyridoxal phosphate will influence gene expression of glycoprotein IIb by interacting with various transcription factors. The result is inhibition of platelet aggregation.

Dietary reference intakes

Life Stage Group	RDA/AI*	UL
Infants 0–6 months 7–12 months	(mg/day) 0.1* 0.3*	(mg/day) ND ND
Children 1-3 yrs 4-8 yrs	0.5 0.6	30 40
Males 9-13 yrs 14-18 yrs 19-50 yrs 50- >70 yrs	1.0 1.3 1.3 1.7	60 80 100 100
Females 9-13 yrs 13-18 yrs 19-50 yrs 50- >70 yrs	1.0 1.2 1.3 1.5	60 80 100 100
Pregnancy <18 yrs 19-50 yrs	1.9 1.9	80 100
Lactation <18 yrs 19-50 yrs	2.0 2.0	80 100

The Institute of Medicine notes that "No adverse effects associated with Vitamin B₆ from food have been reported. This does not mean that there is no potential for adverse effects resulting from high intakes. Because data on the adverse effects of Vitamin B₆ are limited, caution may be warranted. Sensory neuropathy has occurred from high intakes of supplemental forms." See the full Dietary Reference Intake Table [1] from the Institute of Medicine's.

Food sources

Vitamin B₆ is widely distributed in foods in both its free and bound forms. Good sources include meats, whole grain products, vegetables, nuts and bananas. Cooking, storage and processing losses of vitamin B₆ vary and in some foods may be more than 50%, depending on the form of vitamin present in the food. Plant foods lose the least during processing as they contain mostly pyridoxine which is far more stable than the pyridoxal or pyridoxamine found in animal foods. For example, milk can lose 30-70% of its vitamin B₆ content when dried. Vitamin B₆ is found in the germ and aleurone layer of grains and milling results to the reduction of this vitamin in white flour. Freezing and canning are other food processing methods that results in the loss of vitamin B₆ in foods.

Absorption

Vitamin B₆ is absorbed in the jejunum and ileum via passive diffusion. With the capacity for absorption being so great, animals are able to absorb quantities much greater than what is needed for physiological demands. The absorption of pyridoxal phosphate and pyridoxamine phosphate involves their dephosphorylation catalyzed by a membrane-bound alkaline phosphatase. Those products and non-phosphorylated vitamers in the digestive tract are absorbed by diffusion, which is driven by trapping of the vitamin as 5'-phosphates through the action of phosphorylation (by a pyridoxal kinase) in the jejunal mucosa. The trapped pyridoxine and pyridoxamine are oxidized to pyridoxal phosphate in the tissue.

Excretion

The products of vitamin B₆ metabolism are excreted in the urine; the major product of which is 4-pyridoxic acid. It has been estimated that 40-60% of ingested vitamin B₆ is oxidized to 4-pyridoxic acid. Several studies have shown that 4-pyridoxic acid is undetectable in the urine of vitamin B₆ deficient subjects, making it a useful clinical marker to assess the vitamin B₆ status of an individual. Other products of vitamin B₆ metabolism that are excreted in the urine when high doses of the vitamin have been given include pyridoxal, pyridoxamine, and pyridoxine and their phosphates. A small amount of vitamin B₆ is also excreted in the feces.

Deficiencies

The classic clinical syndrome for B₆ deficiency is a seborrhoeic dermatitis-like eruption, atrophic glossitis with ulceration, angular cheilitis, conjunctivitis, intertrigo, and neurologic symptoms of somnolence, confusion, and neuropathy.

While severe vitamin B₆ deficiency results in dermatologic and neurologic changes, less severe cases present with metabolic lesions associated with insufficient activities of the coenzyme pyridoxal phosphate. The most prominent of the lesions is due to impaired tryptophan-niacin conversion. This can be detected based on urinary excretion of xanthurenic acid after an oral tryptophan load. Vitamin B₆ deficiency can also result from impaired transsulfuration of methionine to cysteine. The pyridoxal phosphate-dependent transaminases and glycogen phosphorylase provide the vitamin with its role in gluconeogenesis, so deprivation of vitamin B₆ results in impaired glucose tolerance.

A deficiency of vitamin B₆ alone is relatively uncommon and often occurs in association with other vitamins of the B complex. The elderly and alcoholics have an increased risk of vitamin B₆ deficiency, as well as other micronutrient deficiencies. Renal patients undergoing dialysis may experience vitamin B₆ deficiency. The availability of vitamin B₆ to the body can be affected by certain drugs such as anticonvulsants and corticosteriods.

Clinical assessment of vitamin B₆

Pyridoxal phosphate in the plasma is considered to be one of the best indicator of vitamin B₆ status in the body. When plasma pyridoxal phosphate is less than 10nmol/L, it is indicative of vitamin B₆ deficiency. Urinary 4-pyridoxic acid is also an indicator of vitamin B₆ deficiency. Urinary 4-pyridoxic of less than 3.0 mmol/day is suggestive of vitamin B₆ deficiency.

Toxicity

An overdose of pyridoxine can cause a temporary deadening of certain nerves such as the proprioceptory nerves; causing a feeling of disembodiment common with the loss of proprioception. This condition is reversible when supplementation is stopped.

Because adverse effects have only been documented from vitamin B₆ supplements and never from food sources, this article only discusses the safety of the supplemental form of vitamin B₆ (pyridoxine). Although vitamin B₆ is a water-soluble vitamin and is excreted in the urine, very high doses of pyridoxine over long periods of time may result in painful neurological symptoms known as sensory neuropathy. Symptoms include pain and numbness of the extremities, and in severe cases difficulty walking. Sensory neuropathy typically develops at doses of pyridoxine in excess of 1,000 mg per day. However, there have been a few case reports of individuals who developed sensory neuropathies at doses of less than 500 mg daily over a period of months. None of the studies, in which an objective neurological examination was performed, found evidence of sensory nerve damage at intakes of pyridoxine below 200 mg/day. In order to prevent sensory neuropathy in virtually all individuals, the Food and Nutrition Board of the Institute of Medicine set the tolerable upper intake level (UL) for pyridoxine at 100 mg/day for adults. Because placebo-controlled studies have generally failed to show therapeutic benefits of high doses of pyridoxine, there is little reason to exceed the UL of 100 mg/day.

Oncology

Vitamin B₆ intake is inversely associated with the risk of colorectal cancer.

Preventive roles and therapeutic uses

At least one preliminary study has found that this vitamin may increase dream vividness or the ability to recall dreams. It is thought that this effect may be due to the role this vitamin plays in the conversion of tryptophan to serotonin.

The intake of vitamin B, from either diet or supplements, could cut the risk of Parkinson’s disease by half according to a prospective study from the Netherlands. "Stratified analyses showed that this association was restricted to smokers," wrote the authors.

Pyridoxine has a role in preventing heart disease. Without enough pyridoxine, a compound called homocysteine builds up in the body. Homocysteine damages blood vessel linings, setting the stage for plaque buildup when the body tries to heal the damage. Vitamin B₆ prevents this buildup, thereby reducing the risk of heart attack. Pyridoxine lowers blood pressure and blood cholesterol levels and keeps blood platelets from sticking together. All of these properties work to keep heart disease at bay.

Nutritional supplementation with high dose vitamin B₆ and magnesium is one of the most popular alternative medicine choices for autism.

Some studies suggest that the vitamin B₆-magnesium combination can also help attention deficit disorder, citing improvements in hyperactivity, hyperemotivity/aggressiveness and improved school attention.

A lack of the vitamin may play a role in sensitivity to monosodium glutamate (MSG), a flavor enhancer. This sensitivity can cause headaches, pain and tingling of the upper extremities, nausea, and vomiting. In both of these syndromes, supplementation of pyridoxine alleviates symptoms only when people were deficient in the vitamin to begin with.

If people are marginally deficient in vitamin B₆, they may be more susceptible to carpal tunnel syndrome. Carpal tunnel syndrome is characterized by pain and tingling in the wrists after performing repetitive movements or otherwise straining the wrist on a regular basis. Vitamin B₆ has been shown in at least two small-scale clinical studies to have a beneficial effect on carpal tunnel syndrome, particularly in cases where no trauma or overuse etiology for the CTS is known.

Vitamin B₆ has long been publicized as a cure for premenstrual syndrome (PMS). Study results conflict as to which symptoms are eased, but most of the studies confirm that women who take B₆ supplements have reductions in bloating, breast pain, and premenstrual acne flare, a condition in which pimples break out about a week before a woman's period begins.There is strong evidence that pyridoxine supplementation, starting ten days before the menstrual period, prevents most pimples from forming. This effect is due to the vitamin's role in hormone and prostaglandin regulation. Skin blemishes are typically caused by a hormone imbalance, which vitamin B₆ helps to regulate.

Mental depression is another condition which may result from low vitamin B₆ intake. Because of pyridoxine's role in serotonin and other neurotransmitter production, supplementation often helps depressed people feel better, and their mood improves significantly. It may also help improve memory in older adults. However, the effectiveness as treatment for PMS, PMDD, and clinical depression is debatable.

It is also suggested that ingestion of vitamin B₆ can alleviate some of the many symptoms of an alcoholic hangover and morning sickness from pregnancy. This might be due to B₆'s mild diuretic effect. Though the mechanism is not known, results show that pyridoxamine has a therapeutic effects in clinical trials for diabetic nephropathy.

Larsson et.al. have shown that vitamin B₆ intake and pyridoxal phosphate (PLP) levels are inversely related to the risk of colon cancer. While in their study the correlation with B₆ intake was moderate, it was quite dramatic with PLP levels where the risk of colon cancer was nearly decreased in half.

External links

• Facts about Vitamin B6 from Office of Dietary Supplements at National Institutes of Health
• The B6 database A database of B6-dependent enzymes at University of Parma
• Vitamin B6 Information Sheet from the Linus Pauling Institute at Oregon State University
• Vitamin B6 (and magnesium) in the treatment of autism from the Autism Research Institute
• COT statement on vitamin B6 (pyridoxine) toxicity (June 1997) (Committee on Toxicity of Chemicals in Food, Consumer Products and the Environment (COT))
• Vitamin+B6 at the U.S. National Library of Medicine Medical Subject Headings (MeSH)